Journal: Journal of ethnopharmacology

Article Title: Antiviral effect of fufang yinhua jiedu (FFYH) granules against influenza A virus through regulating the inflammatory responses by TLR7/MyD88 signaling pathway.

doi: 10.1016/j.jep.2021.114063

Figure Lengend Snippet: Fig. 7. In vivo inhibitory effect of FFYH on the inflammatory factors, TNF-α, IL-6, IFN-γ, IP10, and IL-1β caused by influenza a virus (H1N1). The concentration of inflammatory factors in mice sera were quantified with ELISA assays. ##p < 0.05, ###p < 0.01, compared with normal control group; *p < 0.05, **p < 0.01, compared with the virus-infected group (n = 6).

Article Snippet: ELISA kit for examining TNF-α (EK0529), IL-6 (EK0411), IFN-γ(EK0375), IP-10 (EK0736) and IL-1β (EK0394) were obtained from Boster (Wuhan, China).

Techniques: In Vivo, Virus, Concentration Assay, Enzyme-linked Immunosorbent Assay, Control, Infection

Journal: Frontiers in Cell and Developmental Biology

Article Title: lncRNA Gm16410 Mediates PM 2 . 5 -Induced Macrophage Activation via PI3K/AKT Pathway

doi: 10.3389/fcell.2021.618045

Figure Lengend Snippet: Macrophage activation caused inflammation of lung tissue in response to PM 2 . 5 . (A) Immunohistochemistry detection of the macrophage marker F4/80 in mouse lung tissues. Scale bar = 10 μm. (B) Pathological changes in the lung tissues. Scale bar = 10 μm. (C , D) Western blot analysis of TNF-α and IL-1β levels in the lung tissues. The plot gives a quantitative gray-scale analysis of the blot ( n = 3). (E) ELISA analysis of IL-6 in serum ( n = 5). (F) Real-time quantitative PCR analysis of IL-6, TNF-α, and IL-1β in the lung ( n = 8). (G , H) Western blot analysis of the P-PI3K, P-AKT, and NF-κB in mouse lung tissues. The plot gives a quantitative gray-scale analysis of the blot ( n = 3). (I) Immunohistochemistry detection of P-AKT and NF-κB in mouse lung tissues. Scale bar = 10 μm. Data are the means ± SEMs from at least three independent experiments, each performed in triplicate. * P < 0.5 and ** P < 0.01—levels of significance that are different from the control group at the levels, respectively.

Article Snippet: Mouse IL-6 ELISA kit was purchased from Boster (Wuhan, China).

Techniques: Activation Assay, Immunohistochemistry, Marker, Western Blot, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: Astragalus polysaccharide attenuates LPS‐related inflammatory osteolysis by suppressing osteoclastogenesis by reducing the MAPK signalling pathway

doi: 10.1111/jcmm.16683

Figure Lengend Snippet: APS suppressed osteolysis in vivo. (A) Micro‐CT scanning and 3D reconstruction of the outer surface of calvaria in the blank control, LPS control, low‐dose APS‐treated and high‐dose APS‐treated groups. Quantitative analyses were performed to determine the percentage of the resorption area of the whole calvariae (B) and the BV/TV% of the whole calvariae (C). (D) H&E staining was performed on the mouse calvariae collected from the blank control, LPS control, low‐dose APS‐treated and high‐dose APS‐treated groups. (E‐G) The levels of TNF‐α, IL‐1β and IL‐6 in mouse serum were assessed by ELISA. Scale bar, 100 μm. The results are presented as mean ± SD. * P values less than .05, ** P values less than .01 and *** P values less than .001 compared with the controls induced by LPS

Article Snippet: ELISA kits for mouse IL‐6, IL‐1β, and TNF‐α and specific antibodies for CTSK, JNK, p‐JNK, ERK and p‐ERK were obtained from Bioss Antibodies.

Techniques: In Vivo, Micro-CT, Staining, Enzyme-linked Immunosorbent Assay